PCR and Gel Electrophoresis is a lesson by Teach Science with Fergy, containing engaging videos, presentations and activities to help develop students' science knowledge.

PCR and Gel Electrophoresis - Teaching Presentation

PCR and Gel Electrophoresis - Student Presentation

PCR and Gel Electrophoresis - Student Lesson Outline

PCR (Polymerase Chain Reaction)

Join The Amoeba Sisters as they explain the biotechnology technique PCR. This video goes into the basics of how PCR works as well as two examples of its potential use.

Table of Contents:
00:00 Intro
1:34 How does PCR work?
4:31 Why use PCR?
5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19)
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Factual References (and excellent for learning more detail about this topic):

Clark, Mary Ann, et al. “17.1 Biotechnology - Biology 2e | OpenStax.” Biology 2e, OpenStax, 28 Mar. 2018, openstax.org/books/biology-2e/pages/17-1-biotechnology

https://www.fda.gov/consumers/consumer-updates/coronavirus-testing-basics

https://www.fda.gov/media/136151/download

*General length of a primer in base pairs from NIH https://www.genome.gov/genetics-glossary/Primer
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FURTHER READING SUGGESTIONS:

On RT-PCR for SARS-CoV-2:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197457/
https://jamanetwork.com/journals/jama/fullarticle/2765837

On Fluorescent Probes in PCR:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC52277/?page=1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148886/

What about limitations or disadvantages of PCR?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102308/

What about more uses for PCR (as well as more info about Nobel Prize):
https://www.nobelprize.org/prizes/chemistry/1993/press-release/
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The Amoeba Sisters videos demystify science with humor and relevance. The videos center on Pinky's certification and experience in teaching biology at the high school level. Amoeba Sisters videos only cover concepts that Pinky is certified to teach, and they focus on her specialty: secondary life science. Learn more about our videos here: https://www.amoebasisters.com/our-videos

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MUSIC:
Our intro music designed and performed by Jeremiah Cheshire.

End music in this video is listed free to use/no attribution required from the YouTube audio library https://www.youtube.com/audiolibrary/music?feature=blog

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TRANSLATIONS:

Thank you so much to our amazing translators!
Turkish subtitles by Kardelen Ünalleylioğlu
Hindi subtitles by Alisha Aggarwal

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The PCR Song

"Scientists for Better PCR" a Bio-Rad Music Video for the all new 1000-Series Thermal Cyclers

Click below for more information:
http://bio-rad.cnpg.com/lsca/videos/ScientistsForBetterPCR/

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PCR and Gel Electrophoresis - Exit Ticket

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PCR and Gel Electrophoresis

Lesson Description